Infectious Bronchitis Coronavirus Limits Interferon Production by Inducing a Host Shutoff That Requires Accessory Protein 5b.
Identifieur interne : 001004 ( Main/Exploration ); précédent : 001003; suivant : 001005Infectious Bronchitis Coronavirus Limits Interferon Production by Inducing a Host Shutoff That Requires Accessory Protein 5b.
Auteurs : Joeri Kint [Pays-Bas] ; Martijn A. Langereis [Pays-Bas] ; Helena J. Maier [Royaume-Uni] ; Paul Britton [Royaume-Uni] ; Frank J. Van Kuppeveld [Pays-Bas] ; Joseph Koumans [Pays-Bas] ; Geert F. Wiegertjes [Pays-Bas] ; Maria Forlenza [Pays-Bas]Source :
- Journal of virology [ 1098-5514 ] ; 2016.
Descripteurs français
- KwdFr :
- Animaux, Cellules cultivées, Interactions hôte-pathogène, Interféron de type I (antagonistes et inhibiteurs), Poulets, Protéines virales (génétique), Protéines virales (métabolisme), Techniques de knock-out de gènes, Virus de la bronchite infectieuse (génétique), Virus de la bronchite infectieuse (immunologie), Virus de la bronchite infectieuse (pathogénicité), Échappement immunitaire.
- MESH :
- antagonistes et inhibiteurs : Interféron de type I.
- génétique : Protéines virales, Virus de la bronchite infectieuse.
- immunologie : Virus de la bronchite infectieuse.
- métabolisme : Protéines virales.
- pathogénicité : Virus de la bronchite infectieuse.
- Animaux, Cellules cultivées, Interactions hôte-pathogène, Poulets, Techniques de knock-out de gènes, Échappement immunitaire.
English descriptors
- KwdEn :
- Animals, Cells, Cultured, Chickens, Gene Knockout Techniques, Host-Pathogen Interactions, Immune Evasion, Infectious bronchitis virus (genetics), Infectious bronchitis virus (immunology), Infectious bronchitis virus (pathogenicity), Interferon Type I (antagonists & inhibitors), Viral Proteins (genetics), Viral Proteins (metabolism).
- MESH :
- chemical , antagonists & inhibitors : Interferon Type I.
- genetics : Infectious bronchitis virus, Viral Proteins.
- immunology : Infectious bronchitis virus.
- chemical , metabolism : Viral Proteins.
- pathogenicity : Infectious bronchitis virus.
- Animals, Cells, Cultured, Chickens, Gene Knockout Techniques, Host-Pathogen Interactions, Immune Evasion.
Abstract
During infection of their host cells, viruses often inhibit the production of host proteins, a process that is referred to as host shutoff. By doing this, viruses limit the production of antiviral proteins and increase production capacity for viral proteins. Coronaviruses from the genera Alphacoronavirus and Betacoronavirus, such as severe acute respiratory syndrome coronavirus (SARS-CoV), establish host shutoff via their nonstructural protein 1 (nsp1). The Gammacoronavirus and Deltacoronavirus genomes, however, do not encode nsp1, and it has been suggested that these viruses do not induce host shutoff. Here, we show that the Gammacoronavirus infectious bronchitis virus (IBV) does induce host shutoff, and we find that its accessory protein 5b is indispensable for this function. Importantly, we found that 5b-null viruses, unlike wild-type viruses, induce production of high concentrations of type I interferon protein in vitro, indicating that host shutoff by IBV plays an important role in antagonizing the host's innate immune response. Altogether, we demonstrate that 5b is a functional equivalent of nsp1, thereby answering the longstanding question of whether lack of nsp1 in gammacoronaviruses is compensated for by another viral protein. As such, our study is a significant step forward in the understanding of coronavirus biology and closes a gap in the understanding of some IBV virulence strategies.
DOI: 10.1128/JVI.00627-16
PubMed: 27279618
Affiliations:
- Pays-Bas, Royaume-Uni
- Gueldre (province), Utrecht (province)
- Utrecht, Wageningue
- Université d'Utrecht, Université de Wageningue
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Le document en format XML
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Wiegertjes</name><affiliation wicri:level="4"><nlm:affiliation>Cell Biology and Immunology Group, Wageningen Institute of Animal Sciences, Wageningen University, Wageningen, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Cell Biology and Immunology Group, Wageningen Institute of Animal Sciences, Wageningen University, Wageningen</wicri:regionArea><orgName type="university">Université de Wageningue</orgName><placeName><settlement type="city">Wageningue</settlement><region nuts="2">Gueldre (province)</region></placeName></affiliation></author><author><name sortKey="Forlenza, Maria" sort="Forlenza, Maria" uniqKey="Forlenza M" first="Maria" last="Forlenza">Maria Forlenza</name><affiliation wicri:level="4"><nlm:affiliation>Cell Biology and Immunology Group, Wageningen Institute of Animal Sciences, Wageningen University, Wageningen, The Netherlands maria.forlenza@wur.nl.</nlm:affiliation><country wicri:rule="url">Pays-Bas</country><wicri:regionArea>Cell Biology and Immunology Group, Wageningen Institute of Animal Sciences, Wageningen University, Wageningen</wicri:regionArea><orgName type="university">Université de Wageningue</orgName><placeName><settlement type="city">Wageningue</settlement><region nuts="2">Gueldre (province)</region></placeName></affiliation></author></analytic><series><title level="j">Journal of virology</title><idno type="eISSN">1098-5514</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cells, Cultured</term><term>Chickens</term><term>Gene Knockout Techniques</term><term>Host-Pathogen Interactions</term><term>Immune Evasion</term><term>Infectious bronchitis virus (genetics)</term><term>Infectious bronchitis virus (immunology)</term><term>Infectious bronchitis virus (pathogenicity)</term><term>Interferon Type I (antagonists & inhibitors)</term><term>Viral Proteins (genetics)</term><term>Viral Proteins (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Cellules cultivées</term><term>Interactions hôte-pathogène</term><term>Interféron de type I (antagonistes et inhibiteurs)</term><term>Poulets</term><term>Protéines virales (génétique)</term><term>Protéines virales (métabolisme)</term><term>Techniques de knock-out de gènes</term><term>Virus de la bronchite infectieuse (génétique)</term><term>Virus de la bronchite infectieuse (immunologie)</term><term>Virus de la bronchite infectieuse (pathogénicité)</term><term>Échappement immunitaire</term></keywords><keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>Interferon Type I</term></keywords><keywords scheme="MESH" qualifier="antagonistes et inhibiteurs" xml:lang="fr"><term>Interféron de type I</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Infectious bronchitis virus</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines virales</term><term>Virus de la bronchite infectieuse</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Virus de la bronchite infectieuse</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Infectious bronchitis virus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines virales</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Infectious bronchitis virus</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Virus de la bronchite infectieuse</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cells, Cultured</term><term>Chickens</term><term>Gene Knockout Techniques</term><term>Host-Pathogen Interactions</term><term>Immune Evasion</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules cultivées</term><term>Interactions hôte-pathogène</term><term>Poulets</term><term>Techniques de knock-out de gènes</term><term>Échappement immunitaire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">During infection of their host cells, viruses often inhibit the production of host proteins, a process that is referred to as host shutoff. By doing this, viruses limit the production of antiviral proteins and increase production capacity for viral proteins. Coronaviruses from the genera Alphacoronavirus and Betacoronavirus, such as severe acute respiratory syndrome coronavirus (SARS-CoV), establish host shutoff via their nonstructural protein 1 (nsp1). The Gammacoronavirus and Deltacoronavirus genomes, however, do not encode nsp1, and it has been suggested that these viruses do not induce host shutoff. Here, we show that the Gammacoronavirus infectious bronchitis virus (IBV) does induce host shutoff, and we find that its accessory protein 5b is indispensable for this function. Importantly, we found that 5b-null viruses, unlike wild-type viruses, induce production of high concentrations of type I interferon protein in vitro, indicating that host shutoff by IBV plays an important role in antagonizing the host's innate immune response. Altogether, we demonstrate that 5b is a functional equivalent of nsp1, thereby answering the longstanding question of whether lack of nsp1 in gammacoronaviruses is compensated for by another viral protein. As such, our study is a significant step forward in the understanding of coronavirus biology and closes a gap in the understanding of some IBV virulence strategies.</div></front></TEI><affiliations><list><country><li>Pays-Bas</li><li>Royaume-Uni</li></country><region><li>Gueldre (province)</li><li>Utrecht (province)</li></region><settlement><li>Utrecht</li><li>Wageningue</li></settlement><orgName><li>Université d'Utrecht</li><li>Université de Wageningue</li></orgName></list><tree><country name="Pays-Bas"><region name="Gueldre (province)"><name sortKey="Kint, Joeri" sort="Kint, Joeri" uniqKey="Kint J" first="Joeri" last="Kint">Joeri Kint</name></region><name sortKey="Forlenza, Maria" sort="Forlenza, Maria" uniqKey="Forlenza M" first="Maria" last="Forlenza">Maria Forlenza</name><name sortKey="Koumans, Joseph" sort="Koumans, Joseph" uniqKey="Koumans J" first="Joseph" last="Koumans">Joseph Koumans</name><name sortKey="Langereis, Martijn A" sort="Langereis, Martijn A" uniqKey="Langereis M" first="Martijn A" last="Langereis">Martijn A. Langereis</name><name sortKey="Van Kuppeveld, Frank J" sort="Van Kuppeveld, Frank J" uniqKey="Van Kuppeveld F" first="Frank J" last="Van Kuppeveld">Frank J. Van Kuppeveld</name><name sortKey="Wiegertjes, Geert F" sort="Wiegertjes, Geert F" uniqKey="Wiegertjes G" first="Geert F" last="Wiegertjes">Geert F. Wiegertjes</name></country><country name="Royaume-Uni"><noRegion><name sortKey="Maier, Helena J" sort="Maier, Helena J" uniqKey="Maier H" first="Helena J" last="Maier">Helena J. Maier</name></noRegion><name sortKey="Britton, Paul" sort="Britton, Paul" uniqKey="Britton P" first="Paul" last="Britton">Paul Britton</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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